Johns Hopkins All Children's CRIB / MDC

Discovery Metabolomics

MDC offers a variety of different chromatography methods that capture different classes of metabolites (see below).  While databases (retention time indexed) are limited for most chromatography methods, we currently do offer the METLIN workflow, which consists of reverse-phase HPLC followed by MS with positive- and negative-ion mode electrospray (two separate sample injections).  While the METLIN chromatography method is not ideal for profiling of polar metabolites, limitations of this protocol are offset by a large compound database for better putative compound identification (database includes retention time information).

For profiling of polar metabolites the MDC offers a chromatography method (PFPP) which is excellent at resolving a wide variety of hydrophilic compounds that are either positively or negatively charged. For extremely hydrophilic negatively charged compounds, we use ion pairing chromatography. Data is searched against our in-house custom databases for compounds for identity. For putative identification, we search our compounds against the human metabolomics database (HMDB). Accurate mass MS/MS is included in our analysis price. We cannot guarantee that usable MS/MS spectrum will be obtained for each feature.

Users may select which chromatographic method(s) is best suited to their work. As many methods as desired may be used.

Reports are generated from our state-of-the-art analytical software.  Reports are output into an excel spreadsheet and include significant feature differences, putative identifications and links to external databases for more information. The MDC is continuously improving our analytical capabilities.

All services are delivered through the Molecular Determinants Core (MDC), and custom services may be considered on a case-by-case basis by the CRIB. Please contact us for pricing information.